SERUM-INDUCED COLLAGEN GEL CONTRACTION

被引：12

作者：

AKIBA, J

KAKEHASHI, A

UENO, N

TANO, Y

CHAKRABARTI, B

机构：

[1] HARVARD UNIV, SCH MED, SCHEPENS EYE RES INST, DEPT OPHTHALMOL, BOSTON, MA USA

[2] OSAKA UNIV, SCH MED, DEPT OPHTHALMOL, OSAKA, JAPAN

来源：

GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY | 1995年 / 233卷 / 07期

关键词：

D O I：

10.1007/BF00180947

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: To understand the molecular events underlying disease-related Vitreous gel contraction, the effect of serum components on collagen was investigated. Methods: Bovine vitreous or dermal collagen was incubated with a mixture of transglutaminase (TG; factor XIIIa) and fibronectin (FN), and the biochemical changes of collagen were monitored by gel electrophoresis. In addition, serum-induced changes in the volume of the collagen gel were monitored. Results: Gel electrophoresis revealed a new high-molecular-weight band (M(r) 240 000) presumably due to intermolecular cross-links of collagen peptides and FN. The serum components also were shown to cause a significant decrease in the volume of the collagen gel. Conclusion. Collagen gel contraction could be attributed to the collagen-FN-collagen cross-links catalyzed by TG.

引用

页码：430 / 434

页数：5

共 34 条

[1] AGE-RELATED-CHANGES IN THE MOLECULAR-PROPERTIES OF VITREOUS COLLAGEN [J].