HEPATITIS-C VIRUS POLYPROTEIN PROCESSING - KINETICS AND MUTAGENIC ANALYSIS OF SERINE PROTEINASE-DEPENDENT CLEAVAGE

被引：75

作者：

TANJI, Y ^{[1
]}

HIJIKATA, M ^{[1
]}

HIROWATARI, Y ^{[1
]}

SHIMOTOHNO, K ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV VIROL,CHUO KU,TOKYO 104,JAPAN

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 12期

关键词：

D O I：

10.1128/JVI.68.12.8418-8422.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus (HCV) serine proteinase (Cpro-2) is responsible for the processing of HCV nonstructural (NS) protein processing. To clarify the mechanism of Cpro-2-dependent processing, pulse-chase and mutation analyses were performed by using a transient protein production system in cultured cells. Pulse-chase study revealed the sequential production of HCV-NS proteins. Production of p70(NS3) and p66(NS5B) were rapid. An 89-kDa processing intermediate protein (p89) was observed during the early part of the chase. p89 seemed to be cleaved first into a 31-kDa protein (p31) and a p58/56(NS5A). p31 was further processed into p4(NS4A) and p27(NS4B). Mutation analysis of cleavage sites of NS4A/4B, NS4B/5A, and NS5A/5B revealed that cleavage at each site is essentially independent from cleavage occurring at the other site,

引用

页码：8418 / 8422

页数：5

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