PHOTOREDUCTION OF FERREDOXIN AND ITS USE IN NAD(P)+ REDUCTION BY A SUBCELLULAR PREPARATION FROM PHOTOSYNTHETIC BACTERIUM CHLOROBIUM THIOSULFATOPHILUM

被引:59
作者
BUCHANAN, BB
EVANS, MCW
机构
[1] Department of Cell Physiology, University of California, Berkeley
基金
美国国家科学基金会;
关键词
D O I
10.1016/0005-2728(69)90199-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. A subcellular system derived entirely from the photosynthetic green sulfur bacterium, Chlorobium thiosulfatophilum, reduced NAD+ and NADP+ in light-dependent reactions. 2. The rate of NAD+ reduction was twice that of NADP+. 3. The reduction of NAD(P)+ required chlorophyll-containing particles, ferredoxin, and a soluble protein fraction, which catalyzed a reduction of NAD(P)+ with reduced ferredoxin. The soluble protein fraction could be replaced by purified ferredoxin-NADP reductase from spinach chloroplasts. The chlorophyll-containing particles photoreduced ferredoxin with sodium sulfide or 2-mercaptoethanol as the electron donor. 4. The rate of NAD+ reduction was comparable to the over-all rate of CO2 assimilation by C. thiosulfatophilum cells. © 1969.
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页码:123 / &
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