CONSTRUCTION OF BROAD-HOST-RANGE PLASMID VECTORS FOR EASY VISIBLE SELECTION AND ANALYSIS OF PROMOTERS

被引：251

作者：

FARINHA, MA ^{[1
]}

KROPINSKI, AM ^{[1
]}

机构：

[1] QUEENS UNIV, DEPT MICROBIOL & IMMUNOL, KINGSTON K7L 3N6, ONTARIO, CANADA

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 06期

关键词：

D O I：

10.1128/jb.172.6.3496-3499.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have constructed a series of broad-host-range plasmids which use 'visual screens' to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding β-galactosidase and alkaline phosphatase from Escherichia coli and bacterial luciferase from Vibrio harveyi supply the promoterless indicator genes. The constructs were tested in E. coli and Pseudomonas aeruginosa.

引用

页码：3496 / 3499

页数：4

共 25 条

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