HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BETA-PHENYLETHYLAMINE IN HUMAN-PLASMA WITH FLUORESCENCE DETECTION

被引：8

作者：

ISHIDA, J ^{[1
]}

YAMAGUCHI, M ^{[1
]}

NAKAMURA, M ^{[1
]}

机构：

[1] FUKUOKA UNIV,FAC PHARMACEUT SCI,JOHNAN KU,FUKUOKA 81401,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 184卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90016-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and highly sensitive method for the determination of β-phenylethylamine in human plasma is investigated. The method employs high-performance liquid chromatography with fluorescence detection. β-Phenylethylamine and p-methylbenzylamine (internal standard) in human plasma are isolated by cation-exchange chromatography on a Toyopak SP cartridge and then converted into the corresponding fluorescent derivatives with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives are separated within 30 min on a reversed-phase column, TSK gel ODS-120T, with isocratic elution, and detected fluorometrically. The detection limit of β-phenylethylamine is 0.3 pmol/ml in plasma ( S N = 3). © 1990.

引用

页码：86 / 89

页数：4

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