We have cloned DNA sequences extending up to 6000 bp upstream from the first exon of the human prolactin (hPRL) gene. 5000 bp of these upstream sequences were fused to a CAT reporter gene and shown to provide tissue-specific transient expression in rat pituitary GH3 cells. Multihormonal response was found in this transient expression assay, leading to significant 2- to 5-fold induction by addition of 8-chlorophenylthio-cyclic AMP, thyrotropin-releasing hormone, epidermal growth factor, basic fibroblast growth factor, phorbol myristate acetate, a calcium channel agonist (Bay K-8644) and triiodothyronine. A 3-fold inhibition was observed in the presence of the glucocorticoid agonist dexamethasone. The sequence of the hPRL promoter was determined up to coordinate -3470. Computer similarity search between the rat and human sequences showed two highly conserved regions corresponding to the proximal and distal tissue specific enhancers described in both PRL promoters.
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HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLANDHAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLAND
ADAMS, EF
BRAJKOVICH, IE
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HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLANDHAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLAND
BRAJKOVICH, IE
MASHITER, K
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HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLANDHAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLAND
机构:
HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLANDHAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLAND
ADAMS, EF
BRAJKOVICH, IE
论文数: 0引用数: 0
h-index: 0
机构:
HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLANDHAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLAND
BRAJKOVICH, IE
MASHITER, K
论文数: 0引用数: 0
h-index: 0
机构:
HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLANDHAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT MED, ENDOCRINE UNIT, LONDON W12 0HS, ENGLAND