MULTIHORMONAL REGULATION OF THE HUMAN PROLACTIN GENE-EXPRESSION FROM 5000 BP OF ITS UPSTREAM SEQUENCE

被引:68
作者
BERWAER, M
MONGET, P
PEERS, B
MATHYHARTERT, M
BELLEFROID, E
DAVIS, JRE
BELAYEW, A
MARTIAL, JA
机构
[1] UNIV LIEGE,INST CHIM B6,BIOL MOLEC & GENIE GENET LAB,B-4000 SART,BELGIUM
[2] UNIV MANCHESTER,ROYAL INFIRM,DEPT MED,MANCHESTER M13 9PT,LANCS,ENGLAND
基金
英国惠康基金;
关键词
CYCLIC AMP; DEXAMETHASONE; GROWTH FACTOR; THYROTROPIN-RELEASING HORMONE; TRIIODOTHYRONINE;
D O I
10.1016/0303-7207(91)90142-F
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have cloned DNA sequences extending up to 6000 bp upstream from the first exon of the human prolactin (hPRL) gene. 5000 bp of these upstream sequences were fused to a CAT reporter gene and shown to provide tissue-specific transient expression in rat pituitary GH3 cells. Multihormonal response was found in this transient expression assay, leading to significant 2- to 5-fold induction by addition of 8-chlorophenylthio-cyclic AMP, thyrotropin-releasing hormone, epidermal growth factor, basic fibroblast growth factor, phorbol myristate acetate, a calcium channel agonist (Bay K-8644) and triiodothyronine. A 3-fold inhibition was observed in the presence of the glucocorticoid agonist dexamethasone. The sequence of the hPRL promoter was determined up to coordinate -3470. Computer similarity search between the rat and human sequences showed two highly conserved regions corresponding to the proximal and distal tissue specific enhancers described in both PRL promoters.
引用
收藏
页码:53 / 64
页数:12
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