CLONING AND SEQUENCE OF A GENE ENCODING THE L7/L12 RIBOSOMAL-PROTEIN EQUIVALENT OF STREPTOMYCES-ANTIBIOTICUS

被引：8

作者：

PARRA, F ^{[1
]}

BLANCO, G ^{[1
]}

ALONSO, JMM ^{[1
]}

BALBIN, M ^{[1
]}

MENDEZ, C ^{[1
]}

SALAS, JA ^{[1
]}

机构：

[1] UNIV OVIEDO,DEPT BIOL FUNC,E-3306 OVIEDO,SPAIN

来源：

GENE | 1992年 / 118卷 / 01期

关键词：

RECOMBINANT DNA; DIFFERENTIATION; SPORES; COSMID LIBRARY;

D O I：

10.1016/0378-1119(92)90259-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A 50S ribosomal(r) protein from the vegetative mycelium of Streptomyces antibioticus, that is absent or modified in the spore 50S r-subunit, was purified by HPLC. Determination of its N terminus and comparison with amino acid sequence data bases indicated a strong homology with the L7/L12 r-protein from Streptomyces griseus. Screening of a cosmid library of S. antibioticus chromosomal DNA with a 20-mer oligodeoxyribonucleotide probe, corresponding to an internal region of the N terminus, allowed the isolation of two hybridizing clones. A 0.90-kb HindIII-BamHI fragment from one of these clones was sequenced and found to contain a 387-bp open reading frame. The deduced gene product shows clear homology with L7/L12 r-protein equivalent from different bacteria.

引用

页码：127 / 129

页数：3

共 17 条

[1] GENE-EXPRESSION IN STREPTOMYCES - CONSTRUCTION AND APPLICATION OF PROMOTER-PROBE PLASMID VECTORS IN STREPTOMYCES-LIVIDANS [J].