DETERMINATION OF THE COMPLETE COVALENT STRUCTURE OF THE GAMMA(2) SUBUNIT OF BOVINE BRAIN G-PROTEINS BY MASS-SPECTROMETRY

被引：14

作者：

WILCOX, MD ^{[1
]}

SCHEY, KL ^{[1
]}

BUSMAN, M ^{[1
]}

HILDEBRANDT, JD ^{[1
]}

机构：

[1] MED UNIV S CAROLINA, DEPT CELL & MOLEC PHARMACOL & EXPTL THERAPEUT, CHARLESTON, SC 29425 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1979

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete covalent structure of the gamma(2) subunit of bovine brain G proteins was determined by peptide mapping using matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry and by partial sequencing using tandem mass spectrometry. Fragments were identified in proteolytic digests corresponding to all predicted internal sequences of gamma(2), but not for N- or C-terminal sequences. Fragments consistent with modifications of the terminal peptides were observed, however. The presence of these postulated modifications was verified by tandem mass spectrometry. These studies define the structure of the native protein and provide a basis for comparison to expressed proteins. 1995 Academic Press, Inc.

引用

页码：367 / 374

页数：8

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