DETERMINATION OF EQUILIBRIUM CONSTANTS OF ASSOCIATING PROTEIN SYSTEM .4. APPLICATION OF WEIGHT-AVERAGE PARTITION COEFFICIENT TO ANALYSIS OF BM1 NONIDEALITY TERM (AS APPLIED TO BOVINE LIVER L-GLUTAMATE DEHYDROGENASE)

In numerical evaluation of the equilibrium constants of associating protein systems of the type [formula omitted], the more readily obtainable weight-average partition coefficients can be substituted for the quantities [formula omitted] as proposed by Adams (Adams, E. T., Jr. (1967), Biochemistry 6, 1864) and [FORMULA OMITTED] (Adams, E. T., Jr., and Lewis, M. S. (1968), Biochemistry 7, 1044). The resulting equations from a combination of the weight-average partition coefficient and molecular weight data allow a more simplified evaluation of BMi (nonideality term) and the weight fraction of the monomer. Bovine liver L-glutamate dehydrogenase was used in experimental applications. Results confirmed the linear indefinite association described in detail in part III (Chun, P. W., Kim, S. J., Stanley, C. A., and Ackers, G. K. (1969), Biochemistry 8, 1625 (this issue; preceding paper)). © 1969, American Chemical Society. All rights reserved.