EXPRESSION OF DIFFERENT CD8 ISOFORMS ON DISTINCT HUMAN LYMPHOCYTE SUBPOPULATIONS

被引：153

作者：

MOEBIUS, U

KOBER, G

GRISCELLI, AL

HERCEND, T

MEUER, SC

机构：

[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,INST RADIOL & PATHOPHYSIOL,ANGEW IMMUNOL ABT,NEUENHEIMER FELD 280,D-6900 HEIDELBERG,FED REP GER

[2] INST GUSTAVE ROUSSY,DEPT BIOL CELLULAIRE,F-94805 VILLEJUIF,FRANCE

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1991年 / 21卷 / 08期

关键词：

D O I：

10.1002/eji.1830210803

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human CD8+ lymphocyte subpopulations were analyzed for their expression of CD8-alpha and CD8-beta subunits. Investigations with uncloned peripheral blood lymphocytes as well as cloned human natural killer and T cell subpopulations demonstrate that CD3- natural killer cells, T cell receptor gamma/delta, and CD4+CD8+ T cell clones express exclusively CD8-alpha gene products. Structural analysis of CD8 molecules demonstrates that CD8-alpha+/beta- T lymphocytes surface express 75-kDa CD8-alpha/alpha homodimers whereas CD8-alpha/beta lymphocytes express concomittantly two CD8 isoforms of different molecular masses (67 kDa and 75 kDa, respectively). Peptide mapping of these latter two isoforms suggests that CD8 is expressed as alpha/alpha-homodimers and alpha/beta-heterodimers on CD8-alpha-beta+ cells. Importantly, we found that the two CD8 isoforms behave functionally different. Thus, in contrast to CD8-alpha/beta+/CD8-alpha/alpha+ T lymphocytes, cytolytic activity of CD8-alpha/beta-/CD8-alpha/alpha+ T cell clones was not inhibited by anti-CD8 monoclonal antibodies and the latter were not induced to proliferate following CD3/CD8 cross-linking.

引用

页码：1793 / 1800

页数：8

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