ENZYMES FOR CARBOHYDRATE AND PEPTIDE SYNTHESES

被引：13

作者：

WONG, CH

LIU, KKC

KAJIMOTO, T

CHEN, LH

ZHONG, ZY

DUMAS, DP

LIU, JLC

ICHIKAWA, Y

SHEN, GJ

机构：

[1] Department of Chemistry, The Scripps Research Institute, La Jolla, CA, 92037

来源：

PURE AND APPLIED CHEMISTRY | 1992年 / 64卷 / 08期

关键词：

D O I：

10.1351/pac199264081197

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

New practical procedures have been developed for the enzyme-catalyzed synthesis of carbohydrates and peptides. Aldolases have been shown to be effective catalysts for the synthesis of uncommon sugars, particularly azasugars. Enzymatic methods for the large-scale synthesis of oligosaccharides have been developed with the use of glycosyltransferases coupled with the regeneration of sugar nucleotides. Engineered subtilisin variants that are stable and active in anhydrous dimethylformamide and in aqueous solution have been developed for peptide segment coupling.

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页码：1197 / 1202

页数：6

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