SYNTHESIS OF GLUCOSYL-INOSITOL USING A CGTASE, ISOLATION AND CHARACTERIZATION OF THE POSITIONAL ISOMERS, AND ASSIMILATION PROFILES FOR INTESTINAL BACTERIA

被引：14

作者：

SATO, M ^{[1
]}

NAKAMURA, K ^{[1
]}

NAGANO, H ^{[1
]}

YAGI, Y ^{[1
]}

KOIZUMI, K ^{[1
]}

机构：

[1] MUKOGAWA WOMENS UNIV, FAC PHARMACEUT SCI, NISHINOMIYA, HYOGO 663, JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 08期

关键词：

D O I：

10.1007/BF01021638

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel disaccharidc, glucosyl-inositol, was obtained by glucoamylase digestion of the oligoglucosyl-inositols synthesized from myo-inositol as an acceptor and beta-cyclodextrin as a donor by transglucosylation of CGTase from Bacillus ohbensis. The glucosyl-inositol fraction was separated by ion-exchange column chromatography and two positional isomers contained in the fraction were isolated by crystallization and HPLC on a graphitized carbon column. The structure of one of the two isomers isolated was fully determined as 1L(D)-5-O-alpha-D-glucopyranosyl-myo-inositol and another one was presumed as 1D-4-O-alpha-D-glucopyranosyl-myo-inositol from physicochemical data, H-H and C-H COSY NMR analyses and FAB-MS spectra. The disaccharidc was assimilated by Bifidobacterium adolescentis, B. breve, B. infantis and B. longum. On the other hand, it was not utilized by E. coli, Clostridium butyricum, C. clostridiiforme and Klebsiella pneumoniae.

引用

页码：659 / 664

页数：6

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