HOMOGENEITY OF SULFHYDRYL OXIDASE PREPARATIONS OBTAINED BY TRANSIENT COVALENT AFFINITY-CHROMATOGRAPHY

被引:4
作者
JANOLINO, VG
SWAISGOOD, HE
机构
[1] Department of Food Science, Southeast Dairy Foods Research Center, North Carolina State University, Raleigh
关键词
covalent affinity chromatography; immunoglobulin; sulfhydryl oxidase;
D O I
10.3168/jds.S0022-0302(90)78674-2
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Bovine milk sulfhydryl oxidase was prepared by transient covalent affinity chromatography on a cysteinylsuccinamidopropyl glass matrix. The homogeneity of this preparation was examined by immunofixation electrofocusing, SDS-PAGE, and size exclusion chromatography in 6 M guanidinium chloride. The major contaminant of this preparation, prepared from whey diafiltered with a 1000 kdal exclusion limit membrane, was IgG. Immunoglobulin M could not be detected. Presence of IgG in these sulfhydryl oxidase preparations suggests an interaction between IgG and either skim milk membrane vesicles or sulfhydryl oxidase. © 1990, American Dairy Science Association. All rights reserved.
引用
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页码:308 / 313
页数:6
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