RAT-BRAIN PYRUVATE-KINASE - PURIFICATION AND EFFECTS OF LITHIUM

被引：19

作者：

KAJDA, PK ^{[1
]}

BIRCH, NJ ^{[1
]}

OBRIEN, MJ ^{[1
]}

HULLIN, RP ^{[1
]}

机构：

[1] UNIV LEEDS,DEPT BIOCHEM,LEEDS LS2 9LS,W YORKSHIRE,ENGLAND

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1979年 / 11卷 / 04期

关键词：

D O I：

10.1016/S0162-0134(00)80191-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Purified pyruvate kinase was prepared from pooled brains obtained from untreated rats. Its properties suggest that it is similar to type 'M' pyruvate kinase. Lithium inhibition was demonstrated at pharmacologically significant lithium concentrations (7%-12% at 2 mmol 1-1 Li) and this was similar in character to that previously seen in rabbit muscle pyruvate kinase, namely noncompetitive with respect to phosphoenol pyruvate, K+, and Mg2+ but competitive with ADP. © 1979.

引用

页码：361 / 366

页数：6