DOSAGE-DEPENDENT TRANSLATIONAL SUPPRESSION IN YEAST SACCHAROMYCES-CEREVISIAE

The overexpression of SUP35 (SUP2) wild-type gene, caused by increase of its copy number, induces an omnipotent suppression similar to the phenotype of mutants for this gene. The effect of extra-SUP35 was detected for moderate or even low copy number. Moreover, overdosage of the fragment including only the 5'-flanking region and N-terminal 100 bp of protein-coding sequence of SUP35 leads to allosuppression. Multi-SUP35 gene was also incompatible with extrachromosomal suppressor factor-psi. presumably because of a high level of mistranslation. The suppressor effect caused by overdosage of another gene, SUP45 (SUP1), is much lower and can be detected only for one construction which is derived from high copy number plasmid. Suppression induced by extra-SUP35 and especially by extra-SUP45 is affected by the cell environment. A model predicting that the balance of gene products is a key for regulation of translational fidelity is discussed.