ACIDIC-C TERMINUS OF VACCINIA VIRUS DNA-BINDING PROTEIN INTERACTS WITH RIBONUCLEOTIDE REDUCTASE

被引:34
作者
DAVIS, RE
MATHEWS, CK
机构
[1] Dept. of Biochemistry and Biophysics, Oregon State University, Corvallis
关键词
D O I
10.1073/pnas.90.2.745
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Evidence from prokaryotic systems suggests that enzymes of dNTP synthesis are organized near the DNA replication apparatus, allowing direct utilization of dNTPs at their sites of synthesis. To investigate whether similar interactions exist within a eukaryotic environment, we have prepared anti-idiotypic antibodies to the small subunit of vaccinia virus ribonucleotide reductase, and we used these antibodies to search for proteins that interact with this enzyme. This approach identified a 34-kDa viral phosphoprotein, which, like ribonucleotide reductase itself, is localized within infected cells at DNA replication sites. After expression of its structural gene in Escherichia coli, the recombinant protein was purified and found (i) to bind tightly to single-stranded DNA and (ii) to stimulate enzymatic activity of vaccinia ribonucleotide reductase. These observations suggest a physical association between dNTP synthesis and DNA replication in this viral system.
引用
收藏
页码:745 / 749
页数:5
相关论文
共 25 条
[1]   SINGLE-STRANDED-DNA BINDING-PROTEINS REQUIRED FOR DNA-REPLICATION [J].
CHASE, JW ;
WILLIAMS, KR .
ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :103-136
[2]  
DAVIS RE, 1992, THESIS OREGON STATE
[3]   THE COMPLETE DNA-SEQUENCE OF VACCINIA VIRUS [J].
GOEBEL, SJ ;
JOHNSON, GP ;
PERKUS, ME ;
DAVIS, SW ;
WINSLOW, JP ;
PAOLETTI, E .
VIROLOGY, 1990, 179 (01) :247-266
[5]  
HARLOW E, 1988, ANTIBODIES LABORATOR, P641
[6]  
HOWELL ML, 1992, J BIOL CHEM, V267, P1705
[7]   PROTEIN PROTEIN INTERACTIONS WITH THE ACIDIC COOH TERMINUS OF THE SINGLE-STRANDED DNA-BINDING PROTEIN OF THE BACTERIOPHAGE-T4 [J].
KRASSA, KB ;
GREEN, LS ;
GOLD, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :4010-4014
[8]   ARE DNA PRECURSORS CONCENTRATED AT REPLICATION SITES [J].
MATHEWS, CK ;
SINHA, NK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :302-306
[9]   ENZYME ORGANIZATION IN DNA PRECURSOR BIOSYNTHESIS [J].
MATHEWS, CK .
PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 44, 1993, 44 :167-203
[10]   ISOLATION OF 2 DNA-BINDING PROTEINS FROM INTRACELLULAR REPLICATION COMPLEX OF VACCINIA VIRUS [J].
NOWAKOWSKI, M ;
KATES, J ;
BAUER, W .
VIROLOGY, 1978, 84 (02) :260-267