A DATA-BASED REACTION-MECHANISM FOR TYPE-I FRUCTOSE BISPHOSPHATE ALDOLASE

被引：45

作者：

LITTLECHILD, JA

WATSON, HC

机构：

[1] UNIV EXETER,DEPT BIOL SCI,EXETER EX4 4RJ,DEVON,ENGLAND

[2] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1993年 / 18卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0968-0004(93)90048-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structures of three type I fructose-1,6-bisphosphate aldolases have been determined and the common residues surrounding the Schiff base-forming Lys residue located. Armed with this information, it is now possible to propose a mechanism for this ubiquitous enzyme which is consistent with the recorded biochemical data. An interesting, but by no means mandatory, feature of the reaction mechanism is that catalysis can proceed without exchange with the solvent.

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页码：36 / 39

页数：4

相关论文

共 16 条

[1] REACTIVITY OF THIOL GROUPS AND SUBUNIT STRUCTURE OF ALDOLASE [J].

ANDERSON, PJ ;

PERHAM, RN .

BIOCHEMICAL JOURNAL, 1970, 117 (02) :291-&

[2] COMPARATIVE STUDY OF ALDOLASE FROM HUMAN MUSCLE AND LIVER [J].

EAGLES, PAM ;

IQBAL, M .

BIOCHEMICAL JOURNAL, 1973, 133 (03) :429-+

[3] ACTIVITY AND SPECIFICITY OF HUMAN ALDOLASES [J].

GAMBLIN, SJ ;

DAVIES, GJ ;

GRIMES, JM ;

JACKSON, RM ;

LITTLECHILD, JA ;

WATSON, HC .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 219 (04) :573-576

[4] THE CRYSTAL-STRUCTURE OF HUMAN MUSCLE ALDOLASE AT 3.0 A-RESOLUTION [J].

GAMBLIN, SJ ;

COOPER, B ;

MILLAR, JR ;

DAVIES, GJ ;

LITTLECHILD, JA ;

WATSON, HC .

FEBS LETTERS, 1990, 262 (02) :282-286

[5]

HARTMAN FC, 1976, J BIOL CHEM, V251, P3057

[6] THE CRYSTAL-STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE FROM DROSOPHILA-MELANOGASTER AT 2.5 A RESOLUTION [J].

HESTER, G ;

BRENNERHOLZACH, O ;

ROSSI, FA ;

STRUCKDONATZ, M ;

WINTERHALTER, KH ;

SMIT, JDG ;

PIONTEK, K .

FEBS LETTERS, 1991, 292 (1-2) :237-242

[7]

HEYDUK T, 1991, J BIOL CHEM, V266, P15650

[8]

Horecker B. L, 1972, ENZYMES, P213, DOI DOI 10.1016/S1874-6047(08)60450-3

[9] NATURE OF TRANSALDOLASE-DIHYDROXYACETONE COMPLEX [J].

HORECKER, BL ;

CHENG, T ;

PONTREMOLI, S ;

RICCI, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1961, 47 (12) :1949-&

[10] DISTRIBUTION OF FRUCTOSE DIPHOSPHATE ALDOLASE VARIANTS IN BIOLOGICAL SYSTEMS [J].

LEBHERZ, HG ;

RUTTER, WJ .

BIOCHEMISTRY, 1969, 8 (01) :109-&