LAMB AS A CARRIER MOLECULE FOR THE FUNCTIONAL EXPOSITION OF IGG-BINDING DOMAINS OF THE STAPHYLOCOCCUS-AUREUS PROTEIN-A AT THE SURFACE OF ESCHERICHIA-COLI-K12

被引：19

作者：

STEIDLER, L ^{[1
]}

REMAUT, E ^{[1
]}

FIERS, W ^{[1
]}

机构：

[1] STATE UNIV GHENT,MOLEC BIOL LAB,KL LEDEGANCKSTR 35,B-9000 GHENT,BELGIUM

来源：

MOLECULAR & GENERAL GENETICS | 1993年 / 236卷 / 2-3期

关键词：

LAMB; STAPHYLOCOCCAL PROTEIN-A; SURFACE EXPRESSION; IGG BINDING; ESCHERICHIA-COLI;

D O I：

10.1007/BF00277111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One, two or four IgG-binding domains of the Staphylococcus aureus Protein A (SPA) were inserted into the LamB protein which was expressed under control of the tac promoter. The chimeric proteins were shown to be exposed at the cell surface by analysis of isolated outer membranes and also by testing their functional interaction with IgG molecules. We hereby show that the LamB protein can accept as many as 232 amino acids (four SPA domains) and still be incorporated into the Escherichia coli outer membrane, while maintaining the functional conformation of the inserted SPA polypeptides.

引用

页码：187 / 192

页数：6

共 29 条

[1] ANALYSIS OF SIGNALS FOR SECRETION IN THE STAPHYLOCOCCAL PROTEIN-A GENE
ABRAHMSEN, L
MOKS, T
NILSSON, B
HELLMAN, U
UHLEN, M
[J]. EMBO JOURNAL, 1985, 4 (13B) : 3901 - 3906
[2] USE OF OUTER-MEMBRANE PROTEIN PHOE AS A CARRIER FOR THE TRANSPORT OF A FOREIGN ANTIGENIC DETERMINANT TO THE CELL-SURFACE OF ESCHERICHIA-COLI K-12
AGTERBERG, M
ADRIAANSE, H
TOMMASSEN, J
[J]. GENE, 1987, 59 (01) : 145 - 150
[3] BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[4] LINKER MUTAGENESIS IN THE GENE OF AN OUTER-MEMBRANE PROTEIN OF ESCHERICHIA-COLI, LAMB
BOUGESBOCQUET, B
VILLARROYA, H
HOFNUNG, M
[J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1984, 24 (03) : 217 - 228
[5] MUTAGENESIS BY RANDOM LINKER INSERTION INTO THE LAMB GENE OF ESCHERICHIA-COLI-K12
BOULAIN, JC
CHARBIT, A
HOFNUNG, M
[J]. MOLECULAR & GENERAL GENETICS, 1986, 205 (02): : 339 - 348
[6] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI
CASADABAN, MJ
COHEN, SN
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) : 179 - 207
[7] VERSATILITY OF A VECTOR FOR EXPRESSING FOREIGN POLYPEPTIDES AT THE SURFACE OF GRAM-NEGATIVE BACTERIA
CHARBIT, A
MOLLA, A
SAURIN, W
HOFNUNG, M
[J]. GENE, 1988, 70 (01) : 181 - 189
[8] PROBING THE TOPOLOGY OF A BACTERIAL-MEMBRANE PROTEIN BY GENETIC INSERTION OF A FOREIGN EPITOPE - EXPRESSION AT THE CELL-SURFACE
CHARBIT, A
BOULAIN, JC
RYTER, A
HOFNUNG, M
[J]. EMBO JOURNAL, 1986, 5 (11) : 3029 - 3037
[9] FURTHER SEQUENCE-ANALYSIS OF THE PHAGE-LAMBDA RECEPTOR-SITE - POSSIBLE IMPLICATIONS FOR THE ORGANIZATION OF THE LAMB PROTEIN IN ESCHERICHIA-COLI-K12
CHARBIT, A
CLEMENT, JM
HOFNUNG, M
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1984, 175 (03) : 395 - 401
[10] ISOLATION OF DIFFERENT BACTERIOPHAGES USING THE LAMB PROTEIN FOR ADSORPTION ON ESCHERICHIA-COLI K-12
CHARBIT, A
HOFNUNG, M
[J]. JOURNAL OF VIROLOGY, 1985, 53 (02) : 667 - 671

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