COENZYME CONTENT OF PURIFIED ALANINE RACEMASE FROM PSEUDOMONAS

被引：52

作者：

ROSSO, G

TAKASHIMA, K

ADAMS, E

机构：

[1] Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 34卷 / 01期

关键词：

D O I：

10.1016/0006-291X(69)90539-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alanine racemase, induced in Pseudomonas putida by growth on DL-alanine, was purified about 1000-fold to homogeneity. The purified enzyme contains approximately one molar equivalent of pyridoxal phosphate by absorbance, fluorescence, and microbiological assay. No evidence for the presence of a flavin coenzyme was found. © 1969.

引用

页码：134 / +

页数：1

共 23 条

[1]

ADAMS E, 1964, J BIOL CHEM, V239, P1525

[2] GEL-FILTRATION BEHAVIOUR OF PROTEINS RELATED TO THEIR MOLECULAR WEIGHTS OVER A WIDE RANGE [J].