ULTRASTRUCTURAL LOCALIZATION AND DISTRIBUTION OF FREE CHOLESTEROL (3BETA-HYDROXYSTEROLS) IN TISSUES

被引：78

作者：

WILLIAMSON, JR

机构：

[1] Washington University School of Medicine, Department of Pathology, St. Louis

来源：

JOURNAL OF ULTRASTRUCTURE RESEARCH | 1969年 / 27卷 / 1-2期

关键词：

D O I：

10.1016/S0022-5320(69)90023-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method is described for the ultrastructural demonstration of free cholesterol in glutaraldehyde-fixed tissues by the use of digitonin. Characteristic reaction products permit localization of free cholesterol in extracellular as well as intracellular compartments. In numerous cell types, digitonin-induced alterations indicated the presence of cholesterol focally in plasma membranes and in membranes of nuclei, rough endoplasmic reticulum, lysosomes, secretory granules, and cytoplasmic vacuoles. These findings are consistent with the hypothesis that free cholesterol is a widely distributed constituent of cell membranes in general and of surface membranes in particular. © 1969 Academic Press, Inc.

引用

页码：118 / +

页数：1

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