ROLE OF ENVELOPES OF GRAM-NEGATIVE BACTERIA IN CADMIUM BINDING AND TOXICITY

被引：9

作者：

BAUDA, P

BLOCK, JC

机构：

[1] Centre Des Sciences de L'environnement, Metz, 57000, 1, rue des Récollets

来源：

TOXICITY ASSESSMENT | 1990年 / 5卷 / 01期

关键词：

D O I：

10.1002/tox.2540050105

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Whole cells, mureinoplasts, and protoplasts of gram‐negative bacteria were used in cadmium biosorption and toxicity determinations. It was shown that whole cells of Pseudomonas fluorescens bind less metal than other bacterial forms, suggesting the outer membrane acts as a barrier toward metals. Such a role was confirmed by toxicity measurements showing a lower toxic effect of cadmium in the presence of the outer membrane. Chemically modified bacteria, with carboxyl groups masked, were used to identify the cadmium binding sites. Results indicate that care is necessary in interpretation since the acid pH used in the blockage reaction was shown to interfere with the cadmium binding. Moreover, the blockage reagents have been shown to modify the cadmium toxicity. Copyright © 1990 Wiley Periodicals, Inc., A Wiley Company

引用

页码：47 / 60

页数：14

共 30 条

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