NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL ANALYSIS OF THE ESCHERICHIA-COLI AGP GENE ENCODING PERIPLASMIC ACID GLUCOSE-1-PHOSPHATASE

被引：20

作者：

PRADEL, E ^{[1
]}

MARCK, C ^{[1
]}

BOQUET, PL ^{[1
]}

机构：

[1] CENS,DEPT BIOL,SERV BIOCHIM,F-91191 GIF SUR YVETTE,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1128/jb.172.2.802-807.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a calculated M(r) of 43,514. Its promoter was defined by primer extension of the mRNA made in vivo. Like many genes under positive control, its -35 promoter region does not match the consensus. The agp gene is both preceded and followed by transcription termination signals, so it appears to be transcribed as a single unit.

引用

页码：802 / 807

页数：6

共 35 条

[1]

AIBA H, 1981, J BIOL CHEM, V256, P1905

[2]

BEACHAM IR, 1980, J GEN MICROBIOL, V119, P31

[3] GENETIC LOCATION OF GENE (USH) SPECIFYING PERIPLASMIC URIDINE 5'-DIPHOSPHATE GLUCOSE HYDROLASE (5'-NUCLEOTIDASE) IN ESCHERICHIA-COLI K-12 [J].

BEACHAM, IR ;

YAGIL, E .

JOURNAL OF BACTERIOLOGY, 1976, 128 (01) :487-489

[4] CLONING AND CHARACTERIZATION OF THE ESCHERICHIA-COLI GENE CODING FOR ALKALINE-PHOSPHATASE [J].

BERG, PE .

JOURNAL OF BACTERIOLOGY, 1981, 146 (02) :660-667

[5] USE OF TNPHOA TO DETECT GENES FOR EXPORTED PROTEINS IN ESCHERICHIA-COLI - IDENTIFICATION OF THE PLASMID-ENCODED GENE FOR A PERIPLASMIC ACID-PHOSPHATASE [J].

BOQUET, PL ;

MANOIL, C ;

BECKWITH, J .

JOURNAL OF BACTERIOLOGY, 1987, 169 (04) :1663-1669

[6] AMINO-ACID-SEQUENCE OF ESCHERICHIA-COLI ALKALINE-PHOSPHATASE [J].

BRADSHAW, RA ;

CANCEDDA, F ;

ERICSSON, LH ;

NEUMANN, PA ;

PICCOLI, SP ;

SCHLESINGER, MJ ;

SHRIEFER, K ;

WALSH, KA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3473-3477

[7] A COMPUTER ALGORITHM FOR TESTING POTENTIAL PROKARYOTIC TERMINATORS [J].

BRENDEL, V ;

TRIFONOV, EN .

NUCLEIC ACIDS RESEARCH, 1984, 12 (10) :4411-4427

[8] NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL ANALYSIS OF THE ESCHERICHIA-COLI USHA GENE, ENCODING PERIPLASMIC UDP-SUGAR HYDROLASE (5'-NUCLEOTIDASE) - REGULATION OF THE USHA GENE, AND THE SIGNAL SEQUENCE OF ITS ENCODED PROTEIN PRODUCT [J].

BURNS, DM ;

BEACHAM, IR .

NUCLEIC ACIDS RESEARCH, 1986, 14 (10) :4325-4342

[9] CHARACTERIZATION OF THE USH GENE OF ESCHERICHIA-COLI AND ITS PROTEIN PRODUCTS [J].

BURNS, DM ;

ABRAHAM, LJ ;

BEACHAM, IR .

GENE, 1983, 25 (2-3) :343-353

[10]

CHANG CN, 1986, GENE, V44, P121

← 1 2 3 4 →