FACTORS INFLUENCING RETENTION AND RESOLUTION OF SUBSTITUTED ALKYLBENZENES IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY

被引:7
作者
BARMAN, BN [1 ]
MARTIRE, DE [1 ]
机构
[1] GEORGETOWN UNIV,DEPT CHEM,WASHINGTON,DC 20057
关键词
COLUMN LIQUID CHROMATOGRAPHY; ISOMERIC SUBSTITUTED ALKYLBENZENES; RETENTION AND RESOLUTION;
D O I
10.1007/BF02268367
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The retention data of isomeric xylenes, ethyltoluenes and diethylbenzenes, and of mesitylene, benzene, toluene and ethylbenzene were obtained on a reversed-phase column using methanol-water and ethanol-water mobile phases at four different temperatures. This database was used to relate the dependence of solute retention and resolution on the polarity of the mobile phase, solute dipole moment, and column temperature. The additivity of the free energy of the transfer of solute molecules or solute segments from the stationary phase to the mobile phase, was examined for the isomeric compounds. For this, the logarithm of the net retention volume was substituted for the free energy. Deviations from the additivity of free energies indicate that the separation of isomeric substituted alkylbenzenes is governed by their differential interactions with both the polar mobile phase and nonpolar stationary phase. Among the disubstituted alkylbenzenes, ortho-isomers favor the mobile phase more and para-isomers tend to prefer the stationary phase more. The meta-isomers are found to follow the additivity rule closely. These trends are amplified as the polarity of the mobile phase is increased indicating that these isomers are resolved better in water-rich mobile phases. These findings are substantiated by analogous results from gas-liquid chromatographic retention data, estimation of dipole moment effects, and examination of the entropic and enthalpic contributions to the net retention volume.
引用
收藏
页码:347 / 356
页数:10
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