L-DNAS AS POTENTIAL ANTIMESSENGER OLIGONUCLEOTIDES - A REASSESSMENT

被引:80
作者
GARBESI, A
CAPOBIANCO, ML
COLONNA, FP
TONDELLI, L
ARCAMONE, F
MANZINI, G
HILBERS, CW
AELEN, JME
BLOMMERS, MJJ
机构
[1] MENARINI SUD,I-50131 FLORENCE,ITALY
[2] UNIV TRIESTE,DIPARTIMENTO BIOCHIM BIOFIS & CHIM MACROMOLEC,I-34127 TRIESTE,ITALY
[3] LAB BIOPHYS CHEM,6525 ED NIJMEGEN,NETHERLANDS
[4] CIBA GEIGY AG,CH-4002 BASEL,SWITZERLAND
关键词
D O I
10.1093/nar/21.18.4159
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Unnatural L-2'-deoxyribonucleosides L-T, L-dC, L-dA and L-dG were prepared from L-arabinose and assembled, by solution or solid phase synthesis, to give L-oligonucleotides (L-DNAs), which contain all four natural bases. The affinity of these modified oligomers for complementary D-ribo- and D-deoxyribo-oligomers was studied with NMR, UV and CD spectroscopies and mobility shift assay on native PAGE. All experimental results indicate that L-DNAs do not, in general, recognize single-stranded, natural DNA and RNA. Hence, contrary to previous suggestions, it is not possible to envisage their use as wide scope antimessenger agents in the selective control of gene expression.
引用
收藏
页码:4159 / 4165
页数:7
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