KAR3-ENCODED KINESIN IS A MINUS-END-DIRECTED MOTOR THAT FUNCTIONS WITH CENTROMERE-BINDING PROTEINS (CBF3) ON AN IN-VITRO YEAST KINETOCHORE

被引：78

作者：

MIDDLETON, K

CARBON, J

机构：

[1] Department of Biological Sciences, University of California, Santa Barbara

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 15期

关键词：

CHROMOSOME SEGREGATION; MICROTUBULES; MITOSIS;

D O I：

10.1073/pnas.91.15.7212

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have used in vitro motility assays to investigate the mechanism of kinetochore function in the budding yeast Saccharomyces cerevisiae. Functional centromeric DNA plus a tripartite centromere binding protein complex, CBF3, was found to be necessary but not sufficient for in vitro kinetochore activity. A fourth required component was identified as the motor protein Kar3p, a previously reported yeast kinesin known to be involved in karyogamy and mitosis. Our data support genetic evidence suggesting that Kar3p is a kinetochore-associated motor and imply that CBF3 plays a regulatory role in kinetochore function.

引用

页码：7212 / 7216

页数：5

共 27 条

[1] THE CENTROMERE FRONTIER - KINETOCHORE COMPONENTS, MICROTUBULE-BASED MOTILITY, AND THE CEN-VALUE PARADOX [J].