PROPERTIES OF YERSINIA-ENTEROCOLITICA PORINS - INTERFERENCE WITH BIOLOGICAL FUNCTIONS OF PHAGOCYTES, NITRIC-OXIDE PRODUCTION AND SELECTIVE CYTOKINE RELEASE

被引：27

作者：

TUFANO, MA ^{[1
]}

ROSSANO, F ^{[1
]}

CATALANOTTI, P ^{[1
]}

LIGUORI, G ^{[1
]}

MARINELLI, A ^{[1
]}

BARONI, A ^{[1
]}

MARINELLI, P ^{[1
]}

机构：

[1] UNIV NAPLES 2,FAC MED,IST IGIENE,I-80138 NAPLES,ITALY

来源：

RESEARCH IN MICROBIOLOGY | 1994年 / 145卷 / 04期

关键词：

PORIN; VIRULENCE; YERSINIA ENTEROCOLITICA; MONOCYTE; PHAGOCYTOSIS; PHAGOSOME-LYSOSOME FUSION; IMMUNOMODULATION; GRANULOCYTE; CHEMOTAXIS; NYLON WOOL ADHERENCE; CHEMILUMINESCENCE; NO; TNF-ALPHA; IL1-ALPHA; IL6; IL8; IFN-GAMMA; GM-CSF; IL3; IL4;

D O I：

10.1016/0923-2508(94)90185-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have extracted and purified Yersinia enterocolitica ATCC 9610 porins that have molecular weights of 36-38 kDa. They inhibited phagocytosis and phagosome-lysosome fusion (30 %) in human monocytes and caused enhanced nitrite production. Preincubation of polymorphonuclear neutrophils with porins (1-10 mu g/ml/10(6) cells) induced a reduction in chemotaxis, adherence to nylon wool and chemiluminescence. Human lymphomonocytes treated with Y. enterocolitica porins showed a distinctive cytokine profile. Interleukin-1 alpha, interleukin-6 and tumour necrosis factor alpha were released within 3-6 h, while interleukin-8, gamma interferon and granulocyte-macrophage colony stimulating factor were released after 18 h. Interleukin-3 and interleukin-4 were not detected at up to 48 h of incubation. In conclusion, these immunomodulating and histotropic properties may account for Y. enterocolitica infection and its sequelae.

引用

页码：297 / 307

页数：11

共 52 条

[1]

ALBINA JE, 1991, J IMMUNOL, V147, P144

[2] LYMPHOCYTES-T MEDIATE PROTECTION AGAINST YERSINIA-ENTEROCOLITICA IN MICE - CHARACTERIZATION OF MURINE T-CELL CLONES SPECIFIC FOR Y-ENTEROCOLITICA [J].