METHOD FOR ISOLATION OF MATING LESS MUTANTS FROM RHODOSPORIDIUM-TORULOIDES M 1057, A STRAIN OF MATING TYPE-A

被引:9
作者
ABE, K [1 ]
TSUCHIYA, E [1 ]
FUKUI, S [1 ]
KODAMA, N [1 ]
KUSANAGI, A [1 ]
机构
[1] YOKOHAMA CITY UNIV, FAC SCI, KANAGAWA KU, YOKOHAMA, KANAGAWA 232, JAPAN
关键词
D O I
10.2323/jgam.24.287
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A cell suspension was irradiated with UV for 3 min and a segregation culture performed on a yeast-sucrose agar (YS) for 15 h. Segregates were plated on YS agar and incubated for 5 days. Colonies were replicated on minimal agar medium and colonies not giving brown hyphal colonies were isolated as mating-less mutants. Strains (97) were isolated and grouped by mating tube-forming ability and mating ability.
引用
收藏
页码:287 / 290
页数:4
相关论文
共 7 条