DETERMINATION OF D-GLUCOSE IN UNDILUTED WHOLE-BLOOD USING CHEMICALLY MODIFIED ELECTRODES AND SEGMENTED SAMPLE INJECTION IN A FLOW SYSTEM

被引：7

作者：

BUCHRASMUSSEN, T ^{[1
]}

机构：

[1] RADIOMETER MED A-S,DK-2400 COPENHAGEN,DENMARK

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 237卷 / 02期

关键词：

Blood; Enzyme reactor; Glucose;

D O I：

10.1016/S0003-2670(00)83944-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow system for the determination of d-glucose in undiluted whole blood, in which segmented sample injection and on-line dialysis are used to decrease the red cell volume fraction (haematrocrit) dependence, is described. Glucose is degraded enzymatically by means of immobilized glucose dehydrogenase. The nicotinamide coenzyme (NAD+) that is present in the solution is reduced in the enzymatic reaction and is reoxidized amperometically at 0 mV vs. Ag/AgCl on a graphite electrode, modified with phenoxazinium ion. The potential use of the system for clinical analysis is evaluated. © 1990.

引用

页码：405 / 411

页数：7