P-31 NUCLEAR-MAGNETIC-RESONANCE EVIDENCE FOR DIFFERENCES IN INTRACELLULAR PH IN THE ROOTS OF MAIZE SEEDLINGS GROWN WITH NITRATE OR AMMONIUM

被引：43

作者：

GERENDAS, J ^{[1
]}

RATCLIFFE, RG ^{[1
]}

SATTELMACHER, B ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT PLANT SCI,OXFORD OX1 3PF,ENGLAND

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1990年 / 137卷 / 01期

关键词：

!sup]31[!/sup]P NMR; Cytoplasmic pH; nitrogen nutrition; vacuolar pH; Zea mays L;

D O I：

10.1016/S0176-1617(11)80023-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

31P nuclear magnetic resonance (NMR) spectroscopy was used to measure the cytoplasmic and vacuolar pH values in maize (Zea mays) root tissues from seedlings grown in nutrient solutions at different pH values with either nitrate or ammonium as the source of nitrogen. The nitrogen form had little effect on intracellular pH at pH 6, but differences were found at pHs 4 and 8 with the largest effects occurring in root tips with ammonium supply. Intracellular pH in roots was regulated more tightly in the nitrate grown plants than in the ammonium grown plants and it was concluded that the influx of free ammonia at pH 8 and the unfavourable energetics of H+ excretion at low external pH were responsible for this difference. © 1990, Gustav Fischer Verlag, Stuttgart. All rights reserved.

引用

页码：125 / 128

页数：4

共 19 条

[1] INTRACELLULAR PH REGULATION IN RICINUS-COMMUNIS GROWN WITH AMMONIUM OR NITRATE AS N SOURCE - THE ROLE OF LONG-DISTANCE TRANSPORT [J].