HEAT-LABILE ENTEROTOXIN CRYSTAL FORMS WITH VARIABLE A/B5 ORIENTATION - ANALYSIS OF CONFORMATIONAL FLEXIBILITY

被引：12

作者：

SIXMA, TK ^{[1
]}

AGUIRRE, A ^{[1
]}

VANSCHELTINGA, ACT ^{[1
]}

WARTNA, ES ^{[1
]}

KALK, KH ^{[1
]}

HOL, WGJ ^{[1
]}

机构：

[1] UNIV GRONINGEN, BIOSON RES INST, NIJENBORGH 4, 9747 AG GRONINGEN, NETHERLANDS

来源：

FEBS LETTERS | 1992年 / 305卷 / 02期

关键词：

HEAT-LABILE ENTEROTOXIN; TOXIN; CONFORMATIONAL CHANGE;

D O I：

10.1016/0014-5793(92)80869-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new native crystal form of heat-labile enterotoxin (LT) has two AB, complexes in the asymmetric unit with different orientations of the A subunit with respect to the B pentamer. Comparison with other crystal forms of LT shows that there is considerable conformational freedom for orientating the A subunit with respect to the B pentamer. The rotations of A in different crystal forms do not follow one specific axis, but most of them share a hinge point, close to the main interaction area between A and B5. Analysis of the two high-resolution structures available shows that these rotations cause verv little change in the actual interactions between A and B5.

引用

页码：81 / 85

页数：5

共 16 条

[1]

BLACK RE, 1986, 11TH NOB C STOCKH, P23

[2] EXTENSION OF MOLECULAR REPLACEMENT - A NEW SEARCH STRATEGY BASED ON PATTERSON CORRELATION REFINEMENT [J].

BRUNGER, AT .

ACTA CRYSTALLOGRAPHICA SECTION A, 1990, 46 :46-57

[3]

BRUNGER AT, 1988, X PLOR MANUAL

[4] ANALYTICAL MOLECULAR-SURFACE CALCULATION [J].

CONNOLLY, ML .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1983, 16 (OCT) :548-558

[5]

FINKELSTEIN RA, 1988, IMMUNOCHEMICAL MOL G, P85

[6] SOLUTION FOR BEST ROTATION TO RELATE 2 SETS OF VECTORS [J].

KABSCH, W .

ACTA CRYSTALLOGRAPHICA SECTION A, 1976, 32 (SEP1) :922-923

[7] CRYSTAL ORIENTATION AND X-RAY PATTERN PREDICTION ROUTINES FOR AREA-DETECTOR DIFFRACTOMETER SYSTEMS IN MACROMOLECULAR CRYSTALLOGRAPHY [J].

MESSERSCHMIDT, A ;

PFLUGRATH, JW .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1987, 20 (04) :306-315

[8]

MOSS J, 1988, ADV ENZYMOL RAMB, V61, P303

[9]

PRONK SE, 1985, J BIOL CHEM, V260, P3580

[10] COMPARISON OF SUPER-SECONDARY STRUCTURES IN PROTEINS [J].

RAO, ST ;

ROSSMANN, MG .

JOURNAL OF MOLECULAR BIOLOGY, 1973, 76 (02) :241-+

← 1 2 →