IMMUNOAFFINITY ISOLATION OF THE SULFATE CONJUGATE OF 4'-HYDROXYPROPRANOLOL FROM PLASMA

被引:4
作者
ELLER, TD [1 ]
WALLE, UK [1 ]
WALLE, T [1 ]
机构
[1] MED UNIV S CAROLINA,DEPT CELL & MOLEC PHARMACOL & EXPTL THERAPEUT,171 ASHLEY AVE,CHARLESTON,SC 29425
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 612卷 / 02期
关键词
D O I
10.1016/0378-4347(93)80180-C
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Selective extraction of sulfate conjugates of basic drugs from biological matrices has been difficult because of their highly polar nature. Immunoaffinity isolation may be the best solution to this analytical problem. This was tested for a model compound, the metabolite 4'-hydroxypropranolol sulfate (HOPS), which was effectively extracted from plasma by a column containing antibodies to the parent drug propranolol. The specificity was very high, giving little interference from the biological material in subsequent high-performance liquid chromatographic analysis with fluorometric detection. The method for HOPS was highly reproducible and provided a sensitivity of 1 ng/ml plasma. The technique was applied to measurements of HOPS in plasma after therapeutic doses of propranolol as well as to the individual enantiomers after chiral derivatization.
引用
收藏
页码:320 / 325
页数:6
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