KINETOCHORE-STAINING OF SPERMATID MICRONUCLEI - STUDIES OF MICE TREATED WITH X-RADIATION OR ACRYLAMIDE

被引：32

作者：

COLLINS, BW ^{[1
]}

HOWARD, DR ^{[1
]}

ALLEN, JW ^{[1
]}

机构：

[1] ENVIRONM HLTH RES & TESTING INC,RES TRIANGLE PK,NC 27711

来源：

MUTATION RESEARCH | 1992年 / 281卷 / 04期

关键词：

MICRONUCLEUS; KINETOCHORE; SPERMATID; ACRYLAMIDE;

D O I：

10.1016/0165-7992(92)90023-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The rodent spermatid micronucleus (MN) assay was used in conjunction with immunofluorescent techniques to distinguish kinetochores in MN following exposure of mice to X-radiation or acrylamide. After either treatment, modest increases in kinetochore-positive MN were observed. Spermatids which had been exposed during meiotic prophase to X-rays (400 cGy) had approximately 10-fold increases in MN compared to controls; up to 15% of the MN observed were kinetochore-positive. Following acrylamide treatment of meiotic prophase cells, there was a doubling of spermatid MN over baseline levels, approximately one-third of which were kinetochore-positive.

引用

页码：287 / 294

页数：8

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