ULTRASTRUCTURE AND FUNCTION IN SYMPATHETIC-GANGLIA ISOLATED FROM RATS INFECTED WITH PSEUDORABIES VIRUS

1. (1) After inoculation of the pseudorabies virus in the anterior chamber of the eye of the rat, virions can be found only in the neurons of the superior cervical sympathetic ganglion and in the sensory ganglion of the fifth nerve on the inoculated side. Other nervous structures - central or peripheral - are not infected. 2. (2) It is shown that the retrograde axonal flow carries the virus from the eye to the sympathetic neurons. 3. (3) The ultrastructure of the infected neuron has been studied at various intervals after inoculation and at different stages of the viral replication. 4. (4) Excised infected ganglia in vitro show a spontaneous electrophysiological activity that can be recorded on both the post- and preganglionic nerve. Such an activity has never been seen in normal excised ganglion of rat. 5. (5) The shape and frequency of the electrophysiological discharges recorded on the postganglionic nerve have been analyzed at various intervals after inoculation. 6. (6) Correlations established between the ultrastructure, the effect of varios drugs and the electrophysiological activity permit the proposal of various hypothesis about the abnormal activity of the infected neurons. © 1978.