SUBSTANTIALLY INCREASED SENSITIVITY OF THE SPOT-ELISA FOR THE DETECTION OF ANTI-INSULIN ANTIBODY-SECRETING CELLS USING A CAPTURE ANTIBODY AND ENZYME-CONJUGATED INSULIN

被引：14

作者：

VOS, Q ^{[1
]}

CLAASSEN, E ^{[1
]}

BENNER, R ^{[1
]}

机构：

[1] TNO,DEPT IMMUNOL,MED BIOL LAB,RIJSWIJK,NETHERLANDS

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 01期

关键词：

Anti-insulin antibody; Antibody-secreting cell; ELISA; IgG isotype; spot-;

D O I：

10.1016/0022-1759(90)90015-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes an antibody capture spot-ELISA for the detection of anti-insulin antibody-secreting cells. The assay is based on the binding of secreted antibodies by immobilised isotype-specific capture antibodies and subsequent detection of insulin-specific antibodies with a conjugate of human insulin and alkaline phosphatase (HI-AP). Compared with the conventional approach, using antigen for coating and employing an enzyme-linked detecting antibody, this technique improved the detection of murine cells secreting anti-insulin antibodies of different IgG subclasses. © 1990.

引用

页码：89 / 94

页数：6

共 22 条

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