GAS-DIFFUSION DILUTION FLOW-INJECTION METHOD FOR THE DETERMINATION OF ETHANOL IN BEVERAGES WITHOUT SAMPLE PRETREATMENT

被引:47
作者
KUNNECKE, W
SCHMID, RD
机构
[1] GBF - Gesellschaft für Biotechnologische Forschung, Department of Enzyme Technology, D-3300 Braunschweig
关键词
D O I
10.1016/S0003-2670(00)83559-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The combination of immobilized alcohol oxidase (AOD) with a gas-diffusion membrane in a flow-injection system greatly enhanced the specificity of ethanol determination. This gas-diffusion flow-injection system with amperometric detection of hydrogen peroxide had a linear range of analysis from 0.0006 up to 60% (v/v) ethanol. Silicone-modified polypropylene membranes of different thickness were used. Undiluted samples of beer, wine, spirits and medicine could be investigated with excellent correlation with standard methods (r > 0.997). The frequency of analysis was 120-180 samples h-1 and the operational half-life of the immobilized enzyme was 8000 injections within 44 h. © 1990.
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页码:213 / 220
页数:8
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