EFFECT OF DIETARY-SODIUM INTAKE ON INTRACELLULAR CALCIUM IN LYMPHOCYTES OF SALT-SENSITIVE HYPERTENSIVE PATIENTS

被引：34

作者：

ALEXIEWICZ, JM ^{[1
]}

GACIONG, Z ^{[1
]}

PARISE, M ^{[1
]}

KARUBIAN, F ^{[1
]}

MASSRY, SG ^{[1
]}

CAMPESE, VM ^{[1
]}

机构：

[1] UNIV SO CALIF,DEPT MED,DIV NEPHROL,LOS ANGELES,CA 90089

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1992年 / 5卷 / 08期

关键词：

SALT-SENSITIVITY; ESSENTIAL HYPERTENSION; LYMPHOCYTES; CYTOSOLIC CALCIUM;

D O I：

10.1093/ajh/5.8.536

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

High dietary Na+ raises mean arterial pressure (MAP) by more than 10% in salt-sensitive (SS) patients with essential hypertension. To test whether the rise in MAP in these patients is caused by a Na+-linked increase in [Ca2+]i in vascular smooth muscle cells, we measured [Ca2+]i in the lymphocytes of 14 patients with essential hypertension kept on a Na+ intake of 20 mEq/day for 9 days, and 200-mEq/day for 14 days. Nifedipine gastrointestinal transport system (GITS) (30 mg/day) was given during the last 4 days of each diet. We isolated lymphocytes on Ficoll-Hypaque gradient and measured [Ca 2+]i levels using Fura-2 fluorescent dye. During low Na+ intake, there was no difference in MAP (102 +/- 3.5 v 93 +/- 3.8 mm Hg) and in lymphocytes [Ca2+]i (80 +/- 3.0 v 87 +/- 5.4 nmol/L) between the seven salt-sensitive and the seven salt-resistant patients. During high Na+ intake, MAP (92 +/-2.8 mm Hg) and [Ca2+]i (85 +/- 6.8 nmol/L) did not change in salt-resistant patients. On the contrary, MAP (115 +/- 3.4 mm Hg) and [Ca2+]i (130 +/-11.1 nmol/L) increased significantly (P <.01) in the salt-sensitive patients. Nifedipine did not significantly alter MAP and [Ca2+]i in both groups of patients during low Na+ and in salt-resistant patients during high Na+ intake. On the contrary, during high Na+ intake, nifedipine decreased significantly (P <.01) both MAP (104 +/- 2.4 mm Hg) and [Ca2+]i (89 +/- 5.7 nmol/L) in salt-sensitive patients. There was a significant relationship between the changes in [Ca2+]i and in MAP during high Na+ intake or after nifedipine administration (r = 0.7, P <.01). These data demonstrate that high Na+ intake increases [Ca2+]i and MAP in salt-sensitive patients with essential hypertension. Nifedipine reduces MAP more effectively in SS patients, probably by blocking the Na+-linked calcium entry into cells. This suggests the existence of a linkage between the derangements of Na+ and of calcium metabolism in salt-sensitive patients with essential hypertension. The mechanisms of this linkage remain to be established.

引用

页码：536 / 541

页数：6

共 54 条

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