ISOLATION AND CHARACTERIZATION OF THE ALKALINE PROTEASE GENE OF ASPERGILLUS-ORYZAE

被引：38

作者：

MURAKAMI, K ^{[1
]}

ISHIDA, Y ^{[1
]}

MASAKI, A ^{[1
]}

TATSUMI, H ^{[1
]}

MURAKAMI, S ^{[1
]}

NAKANO, E ^{[1
]}

MOTAI, H ^{[1
]}

KAWABE, H ^{[1
]}

ARIMURA, H ^{[1
]}

机构：

[1] KIKKOMAN FOODS INC,DIV RES & DEV,NODA,CHIBA 278,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 11期

关键词：

D O I：

10.1080/00021369.1991.10871048

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The genomic DNA for the alkaline protease (Alp) of the fungus Aspergillus oryzae was isolated using synthetic oligonucleotides as hydridization probes, and the complete nucleotide sequence was identified. The Alp gene is 1374 nucleotides long and contains three introns, one of which is in the pro region and two in the mature coding region. Sequences related to the TATA box (TATAAAT) and the CAAT box (CCAAAT) were found in the 5'-noncoding region. Primer extension analysis showed that three transcriptional start points are present.

引用

页码：2807 / 2811

页数：5

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