CLONING AND EXPRESSION IN STREPTOMYCES-LIVIDANS OF A CHITOSANASE-ENCODING GENE FROM THE ACTINOMYCETE KITASATOSPORIA N174 ISOLATED FROM SOIL

被引：23

作者：

FINK, D ^{[1
]}

BOUCHER, I ^{[1
]}

DENIS, F ^{[1
]}

BRZEZINSKI, R ^{[1
]}

机构：

[1] UNIV SHERBROOKE, FAC SCI, DEPT BIOL, RECH BIOL ACTINOMYCETES GRP, SHERBROOKE J1K 2R1, QUEBEC, CANADA

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 12期

关键词：

D O I：

10.1007/BF01022084

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The chitosanase gene from a new soil isolate, the actinomycete Kitasatosporia N174, was cloned in Streptomyces lividans TK24. The enzyme expressed from the cloned gene had a molecular weight of approximately 29,500; an isoelectric point of 7.5 and was indistinguishable from the purified N174 chitosanase.

引用

页码：845 / 850

页数：6

共 28 条

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