PERIPHERAL SELECTION OF V-DELTA-1+ CELLS WITH RESTRICTED T-CELL RECEPTOR-DELTA GENE JUNCTIONAL REPERTOIRE IN THE PERIPHERAL-BLOOD OF HEALTHY DONORS

To characterize the T cell receptor (TCR) repertoire expressed by the Vdelta1+ gamma/delta T cell population, we have studied the Vdelta1-Jdelta1 junctional sequences from peripheral blood samples of healthy donors. We show that, surprisingly, this repertoire is restricted in most healthy adults, with a donor-specific and relatively stable pattern, whereas this repertoire remains unrestricted in infants, and is similar to that of thymocytes. These data contrast with the general assumption that the junctional repertoire of Vdelta1+ gamma/delta T cells is extensive, and strongly suggest that peripheral recruitment of Vdelta1+ cells bearing particular TCR occurs in humans during the postnatal stage.