DETERMINATION OF KINETIC-PROPERTIES OF SEROTONIN-N-ACETYLTRANSFERASE IN BOVINE PINEAL-GLAND USING HPLC WITH FLUOROMETRIC DETECTION

被引：11

作者：

FAJARDO, N ^{[1
]}

ABREU, P ^{[1
]}

ALONSO, R ^{[1
]}

机构：

[1] UNIV LA LAGUNA,FAC MED,DEPT FISIOL,NEUROENDOCRINOL LAB,APARTADO 55,E-38320 TENERIFE,SPAIN

来源：

JOURNAL OF PINEAL RESEARCH | 1992年 / 13卷 / 02期

关键词：

NAT; ENZYME KINETICS; PINEAL GLAND; HPLC;

D O I：

10.1111/j.1600-079X.1992.tb00058.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The determination of serotonin-N-acetyltransferase (NAT) activity in the bovine pineal gland and other rat tissues was based upon the separation and detection of N-acetyltryptamine formed from tryptamine and acetyl CoA by means of high performance liquid chromatography with fluorimetric detection. In the bovine pineal the enzyme exhibited a Km value of 31.45 +/- 4.89 muM and a Vmax value of 30.90 +/- 1.18 pmol N-acetyltryptamine/min/mg protein for tryptamine, and a Km value of 28.72 +/- 7.50 muM and a Vmax value of 25.90 +/- 1.50 pmol N-acetyltryptamine/min/mg protein for acetyl CoA. The present method is simple, allows the determination of NAT activity from a variety of enzyme sources, has application to pharmacological studies of NAT regulation in tissue cultures. and provides an alternative to current radioenzymatic assays.

引用

页码：80 / 84

页数：5

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