DOUBLE-REDUCTION OF A(1) ABOLISHES THE EPR SIGNAL ATTRIBUTED TO A(1-) - EVIDENCE FOR C2 SYMMETRY IN THE PHOTOSYSTEM-I REACTION-CENTER

被引：37

作者：

HEATHCOTE, P ^{[1
]}

HANLEY, JA ^{[1
]}

EVANS, MCW ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL, DEPT BIOL, LONDON WC1E 6BT, ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1144卷 / 01期

关键词：

PHOTOSYSTEM-I; PHYLLOQUINONE; EPR; REACTION CENTER; (SPINACH);

D O I：

10.1016/0005-2728(93)90030-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Illumination of Photosystem I preparations at room temperature at pH 10.0 in the presence of dithionite has been shown to double-reduce the bound phylloquinone electron acceptor A1 (Setif, P. and Bottin, H. (1989) Biochemistry 28, 2689-2697). The asymmetric EPR signal (g = 2.0048, DELTAH ptp = 0.95 mT) photo-accumulated by illumination of Photosystem I at 205 K is removed by such treatment, confirming the attribution of this EPR signal to A1-. Photoaccumulation of A0- and A1- EPR signals at 230 K in Photosystem I frozen at pH 10.0 approaches a maximum of 4 spins per P700+, suggesting that Photosystem I exhibits the C2 symmetry of other types of photosynthetic reaction centre.

引用

页码：54 / 61

页数：8

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