STERIC EXCLUSION OF CELLS - MECHANISM OF GLYCOSAMINOGLYCAN-INDUCED CELL-AGGREGATION

被引:47
作者
MORRIS, JE
机构
[1] Department of Zoology, Oregon State University, Corvallis
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
D O I
10.1016/0014-4827(79)90545-7
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The mechanism by which chondroitin sulfate enhances both the self-aggregation and the concanavalin A (ConA)-induced agglutination of trypsin-dissociated embryonic chick retina cells was investigated. Studies with fluorescently labeled ConA showed no influence of chondroitin sulfate on patching or capping. When 3H- or 35SO4-labeled glycosaminoglycans or proteoglycans from retinas were added to freshly dissociated cells, an average of less than 2% of the label became associated with the unwashed cell pellet, and most of this was removed from the cells by a single wash. The presence of ConA did not alter the amount of binding by this criterion. Rapid cell aggregation in the absence of ConA was promoted by a number of natural and synthetic polymers. Aggregation rate bore a direct relationship to polymer viscosity at low viscosities and was inhibited at high viscosities, apparently due to reduced cell collision frequency. For any given polymer, aggregation was directly related to its molecular weight and concentration. Linear polymers were more effective than branched ones. Neutral polymers were as effective as those which were strongly polyanionic. Stable aggregates of formalin-fixed cells were not promoted by polymers. All of these observations are consistent with the hypothesis that enhancement of retina cell aggregation by physiological concentrations of glycosaminoglycans is due largely to steric exclusion of the cells by the polymer mesh. Although others have shown that glycosaminoglycans probably interact specifically with some cells, the evidence presented here suggests that these macromolecules by virtue of their excluded volumes could also have important non-specific influences on cell migration and cell reorganization during morphogenesis. © 1979.
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页码:141 / 153
页数:13
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