SCREENING FOR POTASSIUM CHANNEL MODULATORS BY A HIGH THROUGH-PUT 86-RUBIDIUM EFFLUX ASSAY IN A 96-WELL MICROTITER PLATE

被引：19

作者：

DANIEL, S ^{[1
]}

MALKOWITZ, L ^{[1
]}

WANG, HC ^{[1
]}

BEER, B ^{[1
]}

BLUME, AJ ^{[1
]}

ZIAI, MR ^{[1
]}

机构：

[1] AMER CYANAMID CO,CNS RES DEPT,DIV MED RES,PEARL RIVER,NY 10965

来源：

JOURNAL OF PHARMACOLOGICAL METHODS | 1991年 / 25卷 / 03期

关键词：

RB-86 EFFLUX ASSAY; POTASSIUM CHANNELS; MICROTITER PLATE;

D O I：

10.1016/0160-5402(91)90009-T

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A rapid and sensitive Rb-86 efflux assay to detect chemical compounds capable of modulating the ATP-dependent potassium (K(ATP)) channel is described. This assay, which is performed in a 96-well microtiter plate, utilizes a substrate adherent cell line as the target, requires a small amount of Rb-86 as the tracer, and is a suitable system for performing the biochemical and pharmacological characterization of the K(ATP)-channel and its activators. Because this assay is amenable to automation, it presents a useful means for high-volume screening of chemical compounds on a routine basis.

引用

页码：185 / 193

页数：9

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