ACTIVATION OF DNA CLEAVAGE BY DYNEMICIN-A IN A B-Z CONFORMATIONAL JUNCTION

被引：20

作者：

ICHIKAWA, A ^{[1
]}

KUBOYA, T ^{[1
]}

AOYAMA, T ^{[1
]}

SUGIURA, Y ^{[1
]}

机构：

[1] KYOTO UNIV,INST CHEM RES,UJI,KYOTO 611,JAPAN

来源：

BIOCHEMISTRY | 1992年 / 31卷 / 29期

关键词：

D O I：

10.1021/bi00144a019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here that the DNA strand scission by dynemicin A is not only sequence-specific but also conformation-specific. The salt-induced B --> Z conformational transition dramatically enhanced the cleavage by dynemicin A in a B-Z junction region. By contrast, the bleomycin-Fe(II) complex, the elsamicin A-Fe(II) complex, and esperamicin Al did not induce any preferential DNA cutting in such a DNA structure. The characteristic hyperreactivity of dynemicin A is observed in (dC-dG)8- and (dC-dG)12-inserted DNAs, but not in (dC-dG)5-inserted DNA. These results suggest value in the use of dynemicin A as proof of the existence of a B-Z junction in vivo and also may aid in understanding the structure of B-Z Junctions.

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页码：6784 / 6787

页数：4

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