LIGHT-INDUCED DEGRADATION OF D2 PROTEIN IN ISOLATED PHOTOSYSTEM-II REACTION CENTER COMPLEX

被引：30

作者：

BARBATO, R ^{[1
]}

FRISO, G ^{[1
]}

DELAURETO, PP ^{[1
]}

FRIZZO, A ^{[1
]}

RIGONI, F ^{[1
]}

GIACOMETTI, GM ^{[1
]}

机构：

[1] CRIBI,I-35121 PADUA,ITALY

来源：

FEBS LETTERS | 1992年 / 311卷 / 01期

关键词：

D2; PROTEIN; PHOTOINHIBITION; PHOTOSYSTEM-II;

D O I：

10.1016/0014-5793(92)81360-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When isolated photosystem II reaction centers from spinach are exposed to photoinhibitory light in the presence of an electron acceptor, breakdown products of the D2 protein at 28, 25, 23, 18, 9, 5 and 4.5 kDa are detected by immunoblotting with a monospecific anti-D2 polyclonal antibody. In a time-course experiment the 23 and 4.5 kDa fragments show a transient appearance, whilst the others are photoaccumulated. The regions of the D2 protein containing the cleavage sites for the 28 and 18 kDa photoinduced fragments have been identified. Significant degradation of D2 takes place only in the presence of an electron acceptor, and breakdown of the protein is partially prevented by serine-type protease inhibitors.

引用

页码：33 / 36

页数：4

共 27 条

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