SODIUM ARSENITE INDUCES CHROMOSOME ENDOREDUPLICATION AND INHIBITS PROTEIN PHOSPHATASE-ACTIVITY IN HUMAN FIBROBLASTS

被引：55

作者：

HUANG, RN ^{[1
]}

HO, IC ^{[1
]}

YIH, LH ^{[1
]}

LEE, TC ^{[1
]}

机构：

[1] ACAD SINICA, INST BIOMED SCI, TAIPEI 11529, TAIWAN

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1995年 / 25卷 / 03期

关键词：

SODIUM ARSENITE; ENDOREDUPLICATION; SPINDLE ASSEMBLY; PHOSPHATASES;

D O I：

10.1002/em.2850250304

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Arsenic, strongly associated with increased risks of human cancers, is a potent clastogen in a variety of mammalian cell systems. The effect of sodium arsenite (a trivalent arsenic compound) on chromatid separation was studied in human skin fibroblasts (HFW). Human fibroblasts were arrested in S phase by the aid of serum starvation and aphidicolin blocking and then these cells were allowed to synchronously progress into G2 phase. Treatment of the G2-enriched HFW cells with sodium arsenite (0-200 mu M) resulted in arrest of cells in the G2 phase, interference with mitotic division, inhibition of spindle assembly, and induction of chromosome endoreduplication in their second mitosis. Sodium arsenite treatment also inhibited the activities of serine/threonine protein phosphatases and enhanced phosphorylation levels of a smell heat shock protein (HSP27). These results suggest that sodium arsenite may mimic okadaic acid to induce chromosome endoreduplication through its inhibitory effect on protein phosphatase activity. (C) 1995 Wiley-Liss, Inc.

引用

页码：188 / 196

页数：9

共 61 条

[1]

AFSHARI CA, 1993, CANCER RES, V53, P1777

[2]

ALBERTS B, 1983, MOL BIOL CELL, P549

[3]

BLACKWELL R. QUENTIN, 1961, JOUR FORMOSAN MED ASSOC, V60, P1143

[4]

BROWN CC, 1983, J NATL CANCER I, V70, P455

[5]

CAIRNS J, 1994, J BIOL CHEM, V269, P9176

[6] ORIGIN OF ARAC-INDUCED ENDOREDUPLICATED CELLS [J].

CALIGO, MA ;

RAINALDI, G .

MUTATION RESEARCH, 1987, 177 (02) :261-265

[7]

CHEN CJ, 1985, CANCER RES, V45, P5895

[8]

Chou I N, 1989, Biomed Environ Sci, V2, P358

[9]

COHEN P, 1989, J BIOL CHEM, V264, P21435

[10] THE BIMG GENE OF ASPERGILLUS-NIDULANS, REQUIRED FOR COMPLETION OF ANAPHASE, ENCODES A HOMOLOG OF MAMMALIAN PHOSPHOPROTEIN PHOSPHATASE-1 [J].

DOONAN, JH ;

MORRIS, NR .

CELL, 1989, 57 (06) :987-996

← 1 2 3 4 5 6 7 →