ISOLATION AND MAPPING OF A COSMID CLONE CONTAINING THE HUMAN NAT-2 GENE

被引：29

作者：

FRANKE, S

KLAWITZ, I

SCHNAKENBERG, E

ROMMEL, B

VANDEVEN, W

BULLERDIEK, J

SCHLOOT, W

机构：

[1] UNIV BREMEN,ZHG,CTR HUMAN GENET & GENET COUNSELLING,D-28359 BREMEN,GERMANY

[2] UNIV LEUVEN,CTR HUMAN GENET,B-3000 LOUVAIN,BELGIUM

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 199卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1192

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The NAT2 gene encodes for a polymorphic arylalkylamine N-acetyltransferase and thus accounts for the human N-acetylation polymorphism. By a NAT2-specific primer set we have screened a human chromosome 8-specific cosmid library. A positive cosmid clone was mapped by fluorescence in situ hybridization to 8p22. The polymerase chain reaction followed by restriction analysis of the PCR product was used to identify allele 2 to be contained in the cosmid clone. (C) 1994 Academic Press, Inc.

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页码：52 / 55

页数：4

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