TISSUE INHIBITOR OF METALLOPROTEINASES-2 (TIMP-2) IN RAT-LIVER CELLS IS INCREASED BY LIPOPOLYSACCHARIDE AND PROSTAGLANDIN E(2)

被引：29

作者：

ROEB, E

ROSEJOHN, S

ERREN, A

EDWARDS, DR

MATERN, S

GRAEVE, L

HEINRICH, PC

机构：

[1] UNIV MAINZ,MED KLIN 1,PATHOPHYSIOL ABT,D-55101 MAINZ,GERMANY

[2] RHEIN WESTFAL TH AACHEN,INST BIOCHEM,D-52057 AACHEN,GERMANY

[3] UNIV CALGARY,DEPT PHARMACOL & THERAPEUT,CALGARY,AB T2N 4N1,CANADA

来源：

FEBS LETTERS | 1995年 / 357卷 / 01期

关键词：

INFLAMMATORY MEDIATOR; TISSUE INHIBITOR OF METALLOPROTEINASE; EXTRACELLULAR MATRIX; FIBROSIS; LIVER; RAT HEPATOCYTE;

D O I：

10.1016/0014-5793(94)01301-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To explore the functional role of TIMP-2 in liver, we determined TIMP-2 mRNA levels in primary rat hepatocytes and in total rat liver, Rat hepatocytes constitutively express TIMP-2 mRNA at a low level, Incubation with dexamethasone, prostaglandin E(2) and a combination of inflammatory cytokines leads to an up-regulation of TIMP-2 mRNA. In rats in vivo we found a dramatic increase of TIMP-2 expression after intraperitoneal injection of lipopolysaccharide. Compared to our previous findings on TIMP-1 we conclude that TIMP-2 mRNA expression is regulated in a distinct and partially opposite manner. Over-production of TIMP-2 could inhibit the activity of metalloproteinases and thus lead to matrix accumulation, Dysregulation of TIMP-2 synthesis might be involved in the development of liver fibrosis.

引用

页码：33 / 36

页数：4

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