ACTION OF DIPHTHERIA-TOXIN DOES NOT DEPEND ON THE INDUCTION OF LARGE, STABLE PORES ACROSS BIOLOGICAL-MEMBRANES

被引：13

作者：

ALDER, GM

BASHFORD, CL

PASTERNAK, CA

机构：

[1] Division of Biochemistry, Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London

来源：

JOURNAL OF MEMBRANE BIOLOGY | 1990年 / 113卷 / 01期

关键词：

cadmium (Cd[!sup]2+[!/sup]); diphtheria toxin; liposomes; planar bilayers; Vero cells;

D O I：

10.1007/BF01869607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vero cells exposed to diphtheria toxin at pH 4.5 leak monovalent cations but not amino acids or phosphorylated metabolites; affected cells do not take up trypan blue. Monovalent cation leakage is inhibited by 1 mmCd2+, but not by 1 mmZn2+ or Ca2+. Cd2+ blocks calcein leakage from liposomes and closes diphtheria toxin-induced channels in lipid bilayers. It is concluded that translocation of the A fragment of diphtheria toxin across biological membranes does not depend on the formation of large stable pores, but that small Cd2+-sensitive pores may play a role. © 1990 Springer-Verlag New York Inc.

引用

页码：67 / 74

页数：8

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