CELL-CYCLE INHIBITION BY MISONIDAZOLE OF HUMAN-CELLS CULTIVATED INVITRO UNDER AEROBIC CONDITIONS

被引:11
作者
LINDMO, T [1 ]
PETTERSEN, EO [1 ]
WIBE, E [1 ]
机构
[1] NORWEGIAN RADIUM HOSP,NORSK HYDROS INST CANC RES,DEPT TISSUE CULTURE,OSLO,NORWAY
关键词
D O I
10.1038/bjc.1979.257
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
By means of flow cytometric recording of DNA histograms and counting of cells in synchronized populations, we have found that misonidazole (MIS) in clinically relevant concentrations induces cell-kinetic changes in human cells (NHIK 3025) cultivated in vitro under aerobic conditions. The effect seems to be a general lengthening of the cell cycle, affecting all phases. However, induction of this effect is phase-dependent, since only cells exposed to MIS during mitosis and/or early G1 will suffer significant cell-cycle prolongation. In exponentially growing populations this effect of MIS leads to a transient increase in the fraction of G1 cells and a corresponding decrease in the fraction of S cells. The possible significance of this effect for the clinical use of MIS is discussed. © 1979, The British Empire Cancer Campaign for Research. All rights reserved.
引用
收藏
页码:755 / 760
页数:6
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